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High-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization of gene-copy number with co-expression of ERAD-related proteins

Jiao, LC; Zhou, QH; Su, ZX; Xu, L; Yan, YJ

Yan, YJ (reprint author), Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China.

PROTEIN EXPRESSION AND PURIFICATION, 2018; 147 (): 1

Abstract

Rhizopus oryzae lipase (ROL) is an important industrial enzyme limited in application due to its low production in native strains. Here, we used a new......

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