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Focused rational iterative site-specific mutagenesis (FRISM)

Li, DY; Wu, Q; Reetz, MT

Wu, Q (corresponding author), Zhejiang Univ, Dept Chem, Hangzhou, Peoples R China.; Reetz, MT (corresponding author), Max Planck Inst Kohlenforsch, Mulheim, Germany.; Reetz, MT (corresponding author), Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China.

ENZYME ENGINEERING AND EVOLUTION: GENERAL METHODS, 2020; 643 (): 225

Abstract

Directed evolution has emerged as the most productive enzyme engineering method, with stereoselectivity playing a crucial role when evolving mutants f......

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