IPD:通过裂殖子表面蛋白-1型icb5-6片段揭示间日疟原虫的遗传多样性

2017-07-16 MedSci MedSci原创

间日疟仍然是导致生活在其流行地区的人们发病和死亡的潜在原因。了解不同地区间日疟原虫的遗传多样性对于研究种群动态和疟原虫溯源是非常有价值的。研究表明，间日疟的PvMSP-1基因是高度多态性的，并已经作为许多间日疟原虫群体研究的标记。本研究的目的是分析PvMSP-1基因icb5-6片段的遗传多样性，并为将来进一步研究间日疟原虫群体结构和追踪临床病例的起源提供更丰富的遗传多态性数据。

近日，国际杂志《Infectious disease of poverty》在线发表一项关于通过裂殖子表面蛋白-1型icb5-6片段揭示间日疟原虫的遗传多样性的研究。

间日疟仍然是导致生活在其流行地区的人们发病和死亡的潜在原因。了解不同地区间日疟原虫的遗传多样性对于研究种群动态和疟原虫溯源是非常有价值的。研究表明，间日疟的PvMSP-1基因是高度多态性的，并已经作为许多间日疟原虫群体研究的标记。本研究的目的是分析PvMSP-1基因icb5-6片段的遗传多样性，并为将来进一步研究间日疟原虫群体结构和追踪临床病例的起源提供更丰富的遗传多态性数据。

研究对在中国收集的95 株间日疟原虫PvMSP-1 icb5-6标记的进行巢式PCR和测序。为了研究PvMSP-1的遗传多样性，使用DnaSP v5，MEGA软件对PvMSP-1 icb5-6片段的95个核苷酸序列进行基因分型和分析。

从中国收集的95 株间日疟原虫分离株为来自世界各地的本地病例或输入病例虫株。研究共获得了95个390〜460bp的序列。将95个序列分为四个等位基因型（Sal I，Belem，R-III和R-IV）和17个独特的单倍型。R-III和SalⅠ是主要的等位基因型。单倍型多样性（Hd）和核苷酸多样性（Pi）为0.729和0.062，表明由于频繁的重组过程和单核苷酸多态性，PvMSP-1的 icb5-6片段具有最高的多态性水平。dN / dS和Tajima的D值都表明PvMSP-1 icb5-6片段在中性选择压力之下。此外，研究还确定了罕见的重组型R-IV型。

这项研究提供了来自世界各地的间日疟原虫株PvMSP-1标记的高遗传多样性数据。这对于丰富间日疟原虫的多态性信息是有价值的，并有助于进一步研究种群动态和对间日疟原虫的来源进行鉴定。

原始出处：

Wei Ruan, Ling-ling Zhang, Yan Feng, et al.Genetic diversity of Plasmodium vivax revealed by the merozoite surface protein-1 icb5-6 fragment.

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2018-04-30 ay2008fy

#遗传多样性#

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2017-12-22 仁心济世

#间日疟#

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2017-07-19 ren1mw

学习一下了！学习一下吧

83 0
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2017-07-18 12498484m46(暂无昵称)

#疟原虫#

68 0
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2017-07-18 neurosurgeon

#多样性#

80 0
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2017-07-17 明天会更好！

不错的，学习了，谢谢分享！

83 0
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2017-07-17 龙胆草

学习谢谢分享

88 0
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time=2017-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=223062, encodeId=485522306210, content=谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Pw2csslt7CukWiabqiblnQAXaDEiaxhu025c3P0XshccTrbvGWBjKvnHIxbK3vqa80P7WGIFuicCFet97TicrNKpgB3TdabxP1vMh/0, createdBy=28851960965, createdName=榄枷檬, createdTime=Mon Jul 17 07:16:54 CST 2017, time=2017-07-17, status=1, ipAttribution=)]
2017-07-17 135****7952平儿

学了。。。。。。。。。。。

84 0
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2017-07-17 清风拂面

学习了谢谢分享

99 0
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content=学了。。。。。。。。。。。, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Neru1qt53a14aACuSzg3TZhkTcsPYVibyHibhTZ5L4k9qIBWiaej4lgomIsGptf8N16E4mTuIq2DVdJXjPjmwZKrLib/0, createdBy=5a0f2055853, createdName=135****7952平儿, createdTime=Mon Jul 17 07:44:59 CST 2017, time=2017-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=223069, encodeId=80552230693f, content=学习了谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=99, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Mon Jul 17 07:24:10 CST 2017, time=2017-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=223062, encodeId=485522306210, content=谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Pw2csslt7CukWiabqiblnQAXaDEiaxhu025c3P0XshccTrbvGWBjKvnHIxbK3vqa80P7WGIFuicCFet97TicrNKpgB3TdabxP1vMh/0, createdBy=28851960965, createdName=榄枷檬, createdTime=Mon Jul 17 07:16:54 CST 2017, time=2017-07-17, status=1, ipAttribution=)]
2017-07-17 榄枷檬

谢谢分享

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