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Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 152 ()

Glucose isomerase (GI) is a key enzyme in the preparation of high fructose corn syrup (HFCS). In this study, a mutant TEGI-M-L38 M/V137 L (TEGI-M2) of......

JIF:3.409

High D-arabitol production with osmotic pressure control fed-batch fermentation by Yarrowia lipolytica and proteomic analysis under nitrogen source perturbation

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 152 ()

D-arabitol, a five-carbon sugar alcohol, is widely used in food and pharmacy industry as a lower calorie sweetener or intermediate. Appropriate osmoti......

JIF:3.409

Biosynthesis of cis-3-hydroxypipecolic acid from L-lysine using an in vivo dual-enzyme cascade

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 154 ()

Cis-3-Hydroxypipecolic acid (cis-3-HyPip) is an important intermediate for the synthesis of GE81112 tetrapeptides, a small family of unusual nonriboso......

JIF:3.409

Enhanced removal of hydrocarbons BTX by light-driven Aspergillus niger ZnS nanobiohybrids

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 157 ()

Benzene, toluene, and xylene (BTX) are volatile aromatic compounds used in industries, however, they are hazardous when released into the environment.......

JIF:3.409

Improvement of catalytic performance of endoglucanase CgEndo from Colletotrichum graminicola by site-directed mutagenesis

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 154 ()

In order to improve the catalytic efficiency of cellulase for more effective utilization of lignocellulose, a novel endoglucanase (CgEndo) from Collet......

JIF:3.409

Improving the decolorization activity of Bacillus pumilus W3 CotA-laccase to Congo Red by rational modification

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 155 ()

Congo Red (CR) is a typical azo dye with highly toxic and carcinogenic properties. This study aimed to improve the decolorization activity of Bacillus......

JIF:3.409

Modification to increase the thermostability and catalytic efficiency of a-L-rhamnosidase from Bacteroides thetaiotaomicron and high-level expression

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 158 ()

The alpha-L-rhamnosidase BtRha from Bacteroides thetaiotao VPI-5482 is a specific enzyme that selectively hydrolyzes the alpha-1,2 glycosidic bond bet......

JIF:3.409

Enhancing the thermostability of D-allulose 3-epimerase from Clostridium cellulolyticum H10 via a dual-enzyme screening system

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 159 ()

D-Allulose 3-epimerase (DAE) is promising to be used for the production of the rare sugar D-allulose in industry. However, the poor thermostability an......

JIF:3.409

Unveiling steps of the TDP degradation pathway in Variovorax paradoxus TBEA6

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 160 ()

Since the role of biobased plastics increases every year, the search for alternatives to petrol-based polymers is very important. Variovorax paradoxus......

JIF:3.409

Directionally modulating the product chain length of an inulosucrase by semi-rational engineering for efficient production of 1-kestose

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 160 ()

Microbial inulosucrase as a transfructosylation tool has been used to produce inulin and inulin-type fructooli-gosaccharides with various polymerizati......

JIF:3.409

Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 153 ()

The catalytically-active inclusion bodies (CatIBs) represent a promising strategy for immobilizing enzyme without additional carriers and chemicals, w......

JIF:3.409

Enhanced biotransformation of the minor ginsenosides in red ginseng extract by Penicillium decumbens beta-glucosidase

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 153 ()

Compound K (C-K) and Rh2, which are present at low levels in ginseng and ginseng extracts, have higher intestinal absorption rates than other ginsenos......

JIF:3.409

Development of a novel expression platform for heterologous protein production via deleting the p53-like regulator Vib1 in Trichoderma reesei

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 155 ()

Trichoderma reesei is widely used as a protein production host due to its high natural capacity to secrete enzymes. Nonetheless, the complexity and ab......

JIF:3.409

Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 154 ()

Bacillus subtilis immobilization in calcium alginate microparticles was investigated using two techniques: droplet microfluidics-based in T-junction g......

JIF:3.409

pH Shock-promoted lysozyme corona for efficient pathogenic infections treatment: Effects of surface chemistry of mesoporous silica nanoparticles and loading method

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 154 ()

The emergence of antibiotic resistant bacteria because of the antibiotics abusement was the motivation to develop the effective alternatives to tradit......

JIF:3.409

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